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Dr. ing. F.L.W. (Frank) Takken

Faculty of Science
Swammerdam Institute for Life Sciences
Photographer: onbekend

Visiting address
  • Science Park 904
  • Room number:C2.214
Postal address
  • Postbus 1210
    1000 BE Amsterdam
  • Research

    Research

     

    Since 2001, my group studies the molecular and biochemical mechanisms underlying disease -resistance and -susceptibility in plants. We want to understand how the immune system of a plant allows it to recognise a pathogen and mount effective defence responses. Furthermore, we want to elucidate how pathogens manipulate their hosts and manage to evade its immune system. 

    As model crop, we use tomato because of its great genetic tools and its importance for Dutch agriculture. Tomato has many natural enemies and one of them is the root-invading soil-borne pathogen Fusarium oxysporumthe causal agent of Fusarium wilt disease. Resistance to fusarium can be conferred by Resistance genes. These genes encode immune receptors that recognize specific proteins secreted by the fungus. Our aim is to understand how NLR-type immune receptors function in disease resistance. Biochemically we have shown that these proteins act as nucleotide-operated molecular switches. In collaboration with the Cann laboratory (Durham UK) we found that these proteins can bind and bend DNA in vivo, and that in planta they bind DNA once activated. In an NWO funded VICI project we study the role of DNA binding by NLR proteins in plant immunity.

    Tomato is one of the model crops used in our lab. One of the main advantages is that is is easy to make crosses

    To read more about my work click here. This link brings you to an "focus" interview from the faculty with me.

    To read more about my VICI grant read here.

    Although Fusarium oxysporum is infamous for being a devastating pathogen, many strains are actually endophytes that enhance plant resilience to biotic and abiotic stresses. Current research aims at resolving how endophytes evade host resistance while boosting plant immunity, and to elucidate how endophytes differ from pathogens. 

    Disease resistance can also be conferred by a lack of compatibility with a given pathogen. To unravel this mechanism, we focus on the identification of compatibility/susceptibility genes of the host by screening for protein targets that are manipulated by a pathogen. Upon infection Fusarium oxysporum secretes many small proteins in the xylem sap of the host. Many of these Six (Secreted In Xylem) proteins, such as Six3, Six5 and Six6, are important for disease development, like.  We use these Six proteins to identify their planttargets and identified genes whose knockout result in increased disease resistance. Identification of these susceptibility genes increases our understanding on how a pathogen manipulates its host and providing new leads to combat diseases 

    Besides tomato, we use Nicotiana benthamiana to transiently express genes and to study the action and location of recombinant proteins. In this leaf the I-2 Resistance gene is co-expressed with a fungal Avr2 genes. A hypersensitive cell death response is induced where both proteins are present.
    Agroinfiltration in N. benthamiana.

    Molecular Plant Pathology home page

    Green life sciences

    Swammerdam Instituut for Life Sciences

  • Research Team

    Group members

     

    Current members of the research team. 

     

    Margarita Simkovicova PhD student - "Combating Vascular Diseases: Identifying the Guardians of the Xylem Sap", Collaboration with ENZA zaden, funding by ENZA and NWO-VICI program

    Thomas Aalders PhD Student- TOPLESS a novel S gene: Unravelling the manipulation of Topless by a conserved effector from Fusarium oxysporum", funding by NWO talent program: 

    Mila Blekemolen PhD student - "Elucidating the molecular mechanism underlying the virulence and avirulence functions of the Fusarium Avr2/Six5 effector pair" Funding from NWO VICI program and UvA.

    Dr Nico Tintor Postdoc - "The role of effector uptake in plant immunity." ollaboration with ENZA zaden, funding by ENZA and NWO-VICI program

     

    Tomato transformation and regeneration.

    Former lab members

     

    Dr Marijn Knip, Postdoc - VICI project: Linking plant immunity and DNA damage. 

    Dr Manon Richard, Postdoc - VICI project: Linking DNA binding of plant NLR proteins to plant immunity.

    Machiel Beijaert, Technician - VICI project: supporting the VICI project in various ways.

    Dr Francisco de Lamo Ruiz, PhD student - ETN Horizon2020 : How do endophytes evade host resistance while enhancing immunity?

    Dr Maria Constantin, PhD student - ETN Horizon202: How do endophytes differ from pathogens? 

    Dr Lingxue Cao, PhD student - CSC grant: Elucidating the molecular mechanism underlying the virulence and avirulence functions of the Fusarium Avr2/Six5 effector pair,

    Dr Xiaotang Di, PhD student - CSC grant : The Avr2 effector protein from Fusarium as a guide to unravel plant innate immunity.

    Dirk-Jan Valkenburg,  Technician: involved in various ongoing projects in the group.

    Dr Hanna Richter, Technician: involved in all ongoing projects in the group.

    ir. Lisong Ma, PhD student: Analysing the intrinsic functions of Avr2 and its perceival by the resistance protein I-2.  

    Dr Fleur Gawehns, PhD student - CBSG2012: Functional characterisation of Fusarium secreted effector proteins in disease and resistance

    Dr ir. Ewa Lukasik,  PhD student -Bioexploit: "Role of the ATPase activity of the NBS domain of R proteins and the identification of interacting proteins.

    drs Vladimir Krasikov, Postdoc -UvA:  Identifying the role of the Xsp10 protein for resistance and disease to Fusarium oxysporum.

    dr. ir. Harrold van den Burg, Postdoc, NWO Veni: Eludidating  the role of SUMO modification in plant defence signalling.

    ing Marianne de Vroomen Technician, involved in most ongoing projects.

    Drs Mobien Kasiem  PhD student: PhD student: Structure and function of tomato disease resistance proteins.

    Dr Gerben van Ooijen  PhD student -NWO open program: Structure and function of tomato disease resistance proteins. 

    Dr Klaas Jan de Vries, Postdoc - CBSG 2008:  Targeted proteomics of signalosomes mediating pathogen resistance. 

    Dr Wladimir Tameling  PhD student: Disease resistance proteins of the NBS-LRR class, molecular switches of plant defence.

    Dr Sergio de la Fuente van Bentem  PhD student: Unfolding plant disease resistance, the involvement of HSP90 and its co-chaperone  PP5 in I-2 mediated signalling.

    Dr Jack Vossen, Postdoc: Characterisation of the I-2 signalosome.

    Dr Sandra Elzinga,  Postdoc: Characterisation of the Mi-1 signalosome.

    Molecular Plant Pathology group

    Swammerdam Insitute for Life Sciences

  • Seminars, patents and publications before 2002

    Invited speaker

    Selection from the last ten years 

    • Molecular aspects of endophyte-mediated resistance induced by Fusarium oxysporum. VISCEA 4thInternational Conference “Plant Biotic Stresses & Resistance Mechanisms IV” February 19-20, 2020 Technische Universität Wien, Wien, Austria
    • Combatting vascular diseases: identifying the guardians of the xylem. December 9 2019 Invited lecture CRAG, Barcelona, Spain
    • Good fungi turning bad – what is the difference between beneficial and pathogenic Fusarium oxysporum strains? 25-28 September 2019, New Zealand Microbial Society Conference. Palmerston North, New Zealand.
    • The Fusarium oxysporum effector Six8 manipulates plant immunity via the transcriptional co-repressors TPL and TPR1. 25 September 2019, AgResearch, Palmerston North, New Zealand.
    • The good, the bad and the ugly: Unraveling the molecular basis of endophyte mediated resistance in tomato triggered by Fusarium oxysporum.  September 20 2019 Invited lecture Copenhagen University, Copenhagen, Denmark
    • The good, the bad and the ugly: Unraveling the molecular basis of endophyte mediated resistance in tomato triggered by Fusarium oxysporum. June 6 2019, Symposium Molecular Plant Pathology; Past and Future, Amsterdam, The Netherlands 
    •  The CC domains of NLR-type pathogen receptors play essential roles in oligomerization, network formation and immune signalling. July 14-18 2019, MPMI XVIII, Glasgow, Scotland
    • The good, the bad and the ugly : Unraveling the molecular basis of endophyte mediated resistance in tomato triggered by Fusarium oxysporum. March 21 2019, Top Lectures IBL-Leiden, the Netherlands
    • The good, the bad and the ugly; unravelling the molecular basis of beneficial, pathogenic and commensal interactions between Fusarium oxysporum and tomato. October 26, 2018. Oxford University, Department of Plant Sciences, UK
    • The good, the bad and the ugly; unravelling the molecular basis of beneficial, pathogenic and commensal interactions between Fusarium oxysporum and tomato. October 10th, 2018. "The XXIVth Minisymposium on Plant Biology", Koln, Germany
    • The Fusarium oxysporum Avr2-Six5 effector pair alters plasmodesmatal exclusion selectivity facilitating cell-to-cell movement of Avr2.  July 2-3, 2018, 3rd International Conference “Plant Biotic Stresses & Resistance Mechanisms” Vienna Austria
    • The good, the bad and the ugly; unravelling the molecular basis of beneficial, pathogenic and commensal interactions between Fusarium oxysporum and tomato.  June 14, 2018 , KEYS symposium.“Harnessing plant-microbe symbiosis to revolutionize agriculture”, Wageningen, NL
    • A tale of two effector proteins -How a pair of fusarium effector proteins manipulates tomato. January 20 2018, Hamburg University. Hamburg, Germany
    • The Fusarium oxysporum Avr2-Six5 effector pair alters the exclusion selectivity of plasmodesmata. December 1, 2017. Massey University, Palmerston North New Zealand.
    • The good, the bad and the ugly - genetic requirements of beneficial, pathogenic and commensal Fusarium oxysporum strains for colonization of tomato plants. 2-23 Nov 2017. NZSM annual conference 2017: our well-being & our microbes. Auckland, New Zealand
    • The Fusarium oxysporum effector Six8 manipulates plant immunity through association with the transcriptional co-repressors TPL and TPR1. 28 August 2017,  Shanghai Center for Plant Stress Biology, Shanghai,  P.R.  China.
    • The Fusarium oxysporum Avr2-Six5 effector pair alters the exclusion selectivity of plasmodesmata and targets PTI signaling. 23 August 2017. Nanjing Agricultural University, Lab of Molecular Plant Virology, Nanjing. P.R.  China.
    • The Fusarium oxysporum Avr2-Six5 effector pair alters exclusion selectivity of plasmodesmata, 12 July 2017, EMBO Workshop; Intracellular Communication in development and disease, Harnack House, Berlin, Germany 
    • Functional analysis of the Fusarium Avr2 effector protein. 24 March 2017, Gregor Mendel Insitute Vienna Austria.
    • The role of Fusarium effectors in virulence and NLR-mediated innate immunity. December 1 2016, Institut für Biologie/Genetik Martin-Luther-Universität,  Halle (Saale), Germany
    • Functional analysis of the Fusarium Avr2 effector protein. October 26  016, Sainsbury Laboratory, Norwich United Kingdom
    • Gene-for-gene resistance - old dogma’s new insights: NLR’s, sentinels of plant immunity. TSL Summer School: Plant Microbe Interactions. 17-28 August 2015. TSL Norwich, United Kingdom
    • The Fusarium oxysporum effector Six8 manipulates plant immunity through association with the transcriptional co-repressors TPL and TPR1. Annual Conference of the SUSTAIN COST Action (FA1208)   17-19 February 2016 Banyuls, France 
    • The role of Fusarium effectors in virulence and NLR-mediated innate immunity.  New Phytologist workshop; “The apoplast as battleground for plant-microbe interactions”. 9 th– 10 st July 2015, Castle Rauischholzhausen, Gießen, Germany
    • The Fusarium oxysporum effector Six8 manipulates plant immunity through association with the transcriptional co-repressors TPL and TPR1. International Conference on “Plant Biotic Stresses & Resistance Mechanisms II” July 2 - 4, 2015 Vienna, Austria
    • The NLR immune receptors I-2 and Rx1 are DNA deforming proteins. 13-14 April 2015 De Wereld, Lunteren, The Netherlands
    • The NLR immune receptors I-2 and Rx1 are DNA deforming proteins. MPI symposium ‘NLR Biology in Plants and Animals’, May 3-6, 2015 Schloss Ringberg Munich, Germany
    • The NLR immune receptors I-2 and Rx1 are DNA deforming proteins. 15 Jan 2015 University of Copenhagen Copenhagen, Denmark
    • The Fusarium oxysporum effector Six8 manipulates plant immunity through association with the transcriptional co-repressors TPL and TPR1. MINI-SYMPOSIUM: Wageningen University 24 Nov 2014, Wageningen Campus - Netherlands
    • The tomato-fusarium pathosystem: a game of hide and seek, 16th IS-MPMI July 2014 Rhodos Greece
    • The Fusarium oxysporum effector Six8 manipulates plant immunity through association with the transcriptional co-repressors TPL and TPR1. COST WORKSHOP: Structure-guided investigation of effector function, action and recognition. 10 – 12 Sept 2014, Bucharest, Romania
    • De wapenwedloop tussen planten en hun belagers, een strijd om leven en dood.  5 March 2014  Spui lezing Amsterdam
    • The role of Fusarium effectors in NLR-mediated innate immunity. XV International Congress on Molecular Plant-Microbe Interactions (IS-MPMI) July 29-August 2, 2012, Kyoto, Japan
    • How to build a pathogen detector; structural basis of NB-LRR function. EMBO practical course 18-29 june 2012 TSL, Norwich, UK
    • The role of Fusarium effectors in NLR-mediated plant innate immunity. 9 April 2012, Plant Biology Department, University of Fribourg, Fribourg Switzerland.
    • The role of pathogen effectors in NLR-mediated plant innate immunity. International Meeting of the Collaborative Research Center SFB 648  "Communication in Plants and their Responsesto the Environment" May 19-22, 2011, Halle (Saale), Germany 
    • Molecular co-evolution in the tomato-Fusarium pathosystem. October 8, 2010, Justus Liebig University , Giessen, Germany
    • Functional analysis of Fusarium oxysporum f.sp. lycopersici effector proteins. DFG funded colloquium: 'Microbial reprogramming of plant cell development' priority project SPP1212 'Plant-Micro' June 2010,  Freising, Germany
    • The arms race between effectors of Fusarium oxysporum and resistance proteins of tomato. Seminar series School of Biological and Biomedical Sciences, May 2010. Durham University, Durham, UK
    • How to resist a resistance protein? British Society for Plant Pathology, Presidential Meeting, 2009, Oxford, UK
    • How to resist tomato resistance proteins? XIV International Congress on Molecular Plant-Microbe Interactions. July 19-23, 2009, Quebec, Canada.
    • Resistance proteins: scouts of the plant innateimmune system. 18th EUCARPIA congress, "Modern Variety Breeding for Present and Future Needs" 9-12 September 2008, Valencia, Spain
    • Structure-function analysis of plant NB-LRR disease resistance proteins. SFB 670 " Structure Function and Evolution of Innate immunity" 22-23 September 2008, Max-Planck-Institut für Züchtungsforschung, Koln, Germany
    • Resistance proteins: scouts of the plant innate immune system. EPS/Bioexploit Summerschool "On the evolution of plant-pathogen interactions: from principles to practices" 18-20 June 2008, Wageningen, the Netherlands.
    • Resistance proteins: scouts of the plant innate immune system.  4th EPSO Conference, 22-26 June 2008 , Presqu'ile de Giens (Côte d'Azur), France
    •  Molecular aspects of I-2 mediated resistanceto Fusarium oxysporum . Seminar series. May 2008, Max-Planck-Institut für Züchtungsforschung, Koln, Germany
    • Function of the Nucleotide Binding Domain for R Proteins. Keystone Symposium on Plant Innate Immunity, Feb 2008 Keystone Resort,  Keystone, Colorado, USA

    Publications arising from my PhD and Postdoc period, for recent papers see Tab "publications"

    Papers <2002

    • C. F. de Jong, F. L. W. Takken , X. Cai, P. J. G. M. de Wit and M. H. A.J. Joosten (2002)Attenuation of Cf -mediated defenseresponses atelevated temperatures correlates withadecrease in elicitor-binding sites.Molecular Plant-Microbe Interactions 15: 10. 1040-9.
    • P. J. G. M. de Wit, B. F. Brandwagt, H. A. Van den Burg, X. Cai, R. A. L. Van der Hoorn, C.F. De Jong, J. van 't Klooster, M. J. D. de Kock, M. Kruijt, W. H. Lindhout, R. Luderer, F. L. W. Takken , N. Westerink, J.J. M. vervoort and M. H. A. J. Joosten (2002) The molecular basis of co-evolution between Cladosporium fulvum  and tomato. Antonie vanLeeuwenhoek 81: 409-412
    • R. Luderer, F. L. W. Takken , P.J. G.m.Witand M. H. A. J. Joosten (2002) Cladosporiumfulvum overcomes Cf-2 -mediated resistance by producing truncated AVR2 elicitor proteins. Molecular Microbiology 45: 3. 875-84.
    • W. I. L. Tameling, S.D. Elzinga,P.S. Darmin, J. H. Vossen, F. L. W. Takken , M.A.Haring and B. J. C. Cornelissen (2002) The tomato R gene products I-2 and Mi-1 are functional ATP binding proteins withATPase activity. Plant Cell 14: 11. 2929-39.
    • X. Cai, F. L. W. Takken , M. H. A. J. Joosten and P.J. G. M. De Wit(2001) Specific recognition of Avr4 and Avr9 result in distinct patternsof hypersensitive cell death in tomato, but similar patterns ofdefence-related geneepxression. Molecular Plant Pathology 2: 2. 77-86
    • B. F. Brandwagt, L. A.Mesbah, F. L. W. Takken , P. L. Laurent, T. J. A. Kneppers, J. Hille and H. J. J. Nijkamp (2000) A longevity assurance gene homolog of tomatomediates resistanceto Alternaria alternata f. sp. lycopersici toxins and fumonisin B1. Proc Natl Acad Sci USA 97:9.4961-6.
    • F.L. W.Takken and M. H. A. J.Joosten (2000) Plant resistance genes: their structure, function and evolution. European Journal of Plant Pathology 106: 8. 699-713
    • F. L. W. Takken, R. Luderer, S. H. E. J. Gabriëls, N.Westerink, R. Lu, P.J.G. M. de Wit and M.H.A.J.Joosten (2000) A functional cloning strategy, based on a binaryPVX-expression vector, toisolate HR-inducing cDNAsof plant pathogens. The Plant Journal 24: 2). 275-283
    • L. A.Mesbah, T.J. A. Kneppers, F. L. W. Takken, P. Laurent,J.Hille and H. J. J. Nijkamp (1999) Geneticand physical analysis of aYAC contig spanning the fungal diseaseresistancelocus Asc of tomato ( Lycopersicon esculentum ). Molecular and General Genetics 261 (1): 50-57
    • F.L. W. Takken , M. Thomas, M.H. A. J. Joosten, C. Golstein, N.Westerink, J. Hille, H. J. J. Nijkamp, P. J. G. M. DeWit and J. D. G. Jones (1999) A second gene at the tomato Cf-4 locus confers resistance to Cladosporium fulvum through recognition of anovel avirulence determinant. The Plant Journal 20: 3. 279-288
    • F.L. W. Takken , D. Schipper, H. J. J. Nijkamp and J.Hille (1998)Identification and Ds-tagged isolation of a new gene at the Cf-4 locus of tomato involved in disease resistance to Cladosporium fulvum race 5. The Plant Journal 14: 401-411

    patent applications

    • Takken, F.L.W., &Wit, P.J.G.M.de (10-01-2002). Elicitor from Cladosporium . no WO02/02787.
    • Turk, S.C.H.J., Takken, F.L.W., Jong, C.F. de, Joosten, M.H.A.J.,& Wit, P.J.G.M. de (02-01-2003). Nucleotide sequences involved in plantdisease resistance.no01202420.4./ WO03000930  
    • Joosten, M.H.A.J., Jong, C.F. de, Wit, P.J.G.M. de, Takken, F.L.W., & Turk, S.C.H.J. (17-03-2004). Nucleotide sequences involved in plantresistance. no EP1397515
  • Biography

    Biography

    Frank Takken (1969) is interested in how plants defend themselves against pathogens. Plants are able to sense the presence of invading microbes. Recognition subsequently results in activation of the plant innate immune system. In this process, resistance proteins play a key role as they mediate pathogen recognition and trigger the activation of downstream signaling cascades that halt the pathogen and thereby prevent disease. An example of a succesfull immune response is shown in the figure below where the tomato  Immunity-2 ( I-2) gene halts ingress of the pathogenic fungus Fusarium oxysporum.

    Resistance genes come in different flavors, but the majority encodes intracellular tri-partite proteins that contain a central nucleotide-binding domain. One of Frank's major research interests is the role the distinct domains in these proteins have for their function. Structure-function analysis in combination with 3D modeling of the different subdomain is used to predict mutations that will have specific effects on the activity of these proteins. These mutants are subsequently analyzed for altered biochemical properties, differences in inter- and intramolecular interactions as well as for their effect on disease resistance.

    To examine how resistance proteins activate downstream signaling it is essential to know their interacting partners. Using various screens, candidate interacting proteins, and recently also DNA, have been identified and these interactors are analyzed for their involvement in plant defense. Part of this research is funded by an NWO VICI grant that was awarded in 2015. Besides resistance genes, I also have a long standing interest to resolve the molecular basis of host susceptibility and the induced resistance responses triggered by biocontrol fungi.

    Frank is appointed as Associate professor in the Molecular Plant Pathology group at the Swammerdam Institute for Life Sciences and was from 2012-2019 Scientific Advisor for Scienza Biotechnology. Previously he worked as postdoc at the Phytopathology group at Wageningen University. During this time he worked on a self-written project at the biotech company Keygene NV in Wageningen. In that project he focused on the identification of genes that are transcriptionally regulated during the onset of plant defense signaling. He received his PhD in 1999 from the Free University of Amsterdam for his pioneering work on the isolation of resistance genes from tomato.

    A resistant tomato plant carrying I-2 (left) and susceptible one (right side) infected with Fusarium oxysporum f.sp. lycopersici.
  • Publications

    2020

    2019

    2018

    2017

    2016

    • Di, X., Gomila, J., Ma, L., van den Burg, H. A., & Takken, F. L. W. (2016). Uptake of the Fusarium Effector Avr2 by Tomato Is Not a Cell Autonomous Event. Frontiers in Plant Science, 7, [1915]. https://doi.org/10.3389/fpls.2016.01915 [details]
    • Di, X., Takken, F. L. W., & Tintor, N. (2016). How Phytohormones Shape Interactions between Plants and the Soil-Borne Fungus Fusarium oxysporum. Frontiers in Plant Science, 7, [170]. https://doi.org/10.3389/fpls.2016.00170 [details]
    • Fenyk, S., Dixon, C. H., Gittens, W. H., Townsend, P. D., Sharples, G. J., Pålsson, L. O., ... Cann, M. J. (2016). The Tomato Nucleotide-binding Leucine-rich Repeat (NLR) Immune Receptor I-2 Couples DNA-Binding to Nucleotide-Binding Domain Nucleotide Exchange. The Journal of Biological Chemistry, 291(3), 1137-1147. https://doi.org/10.1074/jbc.M115.698589 [details]
    • Peng, H. C., Mantelin, S., Hicks, G. R., Takken, F. L. W., & Kaloshian, I. (2016). The Conformation of the Plasma Membrane-Localized SlSERK1 - Mi-1.2 Complex is Altered by a Potato Aphid Derived Effector. Plant Physiology, 171(3), 2211-2222. https://doi.org/10.1104/pp.16.00295 [details]

    2015

    2014

    2013

    2012

    • Bai, S., Liu, J., Chang, C., Zhang, L., Maekawa, T., Wang, Q., ... Shen, Q. H. (2012). Structure-function analysis of barley NLR immune receptor MLA10 reveals its cell compartment specific activity in cell death and disease resistance. PLoS Pathogens, 8(6), [e10027]. https://doi.org/10.1371/journal.ppat.1002752 [details]
    • Lukasik-Shreepaathy, E., Slootweg, E., Richter, H., Goverse, A., Cornelissen, B. J. C., & Takken, F. L. W. (2012). Dual regulatory roles of the extended N terminus for activation of the tomato MI-1.2 resistance protein. Molecular Plant-Microbe Interactions, 25(8), 1045-1057. https://doi.org/10.1094/MPMI-11-11-0302 [details]
    • Lukasik-Shreepaathy, E., Vossen, J. H., Tameling, W. I. L., de Vroomen, M. J., Cornelissen, B. J. C., & Takken, F. L. W. (2012). Protein-protein interactions as a proxy to monitor conformational changes and activation states of the tomato resistance protein I-2. Journal of Experimental Botany, 63(8), 3047-3060. https://doi.org/10.1093/jxb/ers021 [details]
    • Ma, L., Lukasik, E., Gawehns, F., & Takken, F. L. W. (2012). The use of agroinfiltration for transient expression of plant resistance and fungal effector proteins in Nicotiana benthamiana leaves. In M. D. Bolton, & B. P. H. J. Thomma (Eds.), Plant fungal pathogens: methods and protocols (pp. 61-74). (Methods in molecular biology; No. 835). New York: Humana Press. https://doi.org/10.1007/978-1-61779-501-5_4 [details]
    • Takken, F. L. W., & Goverse, A. (2012). How to build a pathogen detector: structural basis of NB-LRR function. Current Opinion in Plant Biology, 15(4), 375-384. https://doi.org/10.1016/j.pbi.2012.05.001 [details]

    2011

    • Krasikov, V., Dekker, H. L., Rep, M., & Takken, F. L. W. (2011). The tomato xylem sap protein XSP10 is required for full susceptibility to Fusarium wilt disease. Journal of Experimental Botany, 62(3), 963-973. https://doi.org/10.1093/jxb/erq327 [details]
    • Maekawa, T., Cheng, W., Spiridon, L. N., Töller, A., Lukasik, E., Saijo, Y., ... Schulze-Lefert, P. (2011). Coiled-coil domain-dependent homodimerization of intracellular barley immune receptors defines a minimal functional module for triggering cell death. CELL Host & Microbe, 9(3), 187-199. https://doi.org/10.1016/j.chom.2011.02.008 [details]
    • Mantelin, S., Peng, H. C., Li, B., Atamian, H. S., Takken, F. L. W., & Kaloshian, I. (2011). The receptor-like kinase SlSERK1 is required for Mi-1-mediated resistance to potato aphids in tomato. Plant Journal, 67(3), 459-471. https://doi.org/10.1111/j.1365-313X.2011.04609.x [details]

    2010

    • Ament, K., Krasikov, V., Allmann, S., Rep, M., Takken, F. L. W., & Schuurink, R. C. (2010). Methyl salicylate production in tomato affects biotic interactions. Plant Journal, 62(1), 124-134. https://doi.org/10.1111/j.1365-313X.2010.04132.x [details]
    • Takken, F., & Rep, M. (2010). The arms race between tomato and Fusarium oxysporum. Molecular Plant Pathology, 11(2), 309-314. https://doi.org/10.1111/j.1364-3703.2009.00605.x [details]
    • Takken, F., van Ooijen, G., Lukasik, E., Ma, L., Gawehns, F., Houterman, P., & Rep, M. (2010). How to resist a tomato resistance gene? In H. Antoun, T. Avis, L. Brisson, D. Prévost, & M. Trepanier (Eds.), Proceedings of the 14th International Congress on Molecular Plant-Microbe Interactions: Quebec City, Quebec, Canada, July 19-23, 2009 (Biology of plant-microbe interactions; Vol. 7). St. Paul, MN: International Society for Molecular Plant-Microbe Interactions. [details]
    • van Ooijen, G., Lukasik, E., van den Burg, H. A., Vossen, J. H., Cornelissen, B. J. C., & Takken, F. L. W. (2010). The small heat shock protein 20 RSI2 interacts with and is required for stability and function of tomato resistance protein I-2. Plant Journal, 63(4), 563-572. https://doi.org/10.1111/j.1365-313X.2010.04260.x [details]
    • van den Burg, H. A., Kini, R. K., Schuurink, R. C., & Takken, F. L. W. (2010). Arabidopsis small ubiquitin-like modifier paralogs have distinct functions in development and defense. The Plant Cell, 22(6), 1998-2016. https://doi.org/10.1105/tpc.109.070961 [details]

    2009

    2008

    • Tameling, W. I. L., & Takken, F. L. W. (2008). Resistance proteins: Scouts of the plant innate immune system. European Journal of Plant Pathology, 121(3), 243-255. https://doi.org/10.1007/s10658-007-9187-8 [details]
    • van Ooijen, G., Mayr, G., Albrecht, M., Cornelissen, B. J. C., & Takken, F. L. W. (2008). Transcomplementation, but not physical association of the CC-NB-ARC and LRR domains of tomato R protein Mi-1.2 is altered by mutations in the ARC2 subdomain. Molecular Plant, 1(3), 401-410. https://doi.org/10.1093/mp/ssn009 [details]
    • van Ooijen, G., Mayr, G., Kasiem, M. M. A., Albrecht, M., Cornelissen, B. J. C., & Takken, F. L. W. (2008). Structure-function analysis of the NB-ARC domain of plant disease resistance proteins. Journal of Experimental Botany, 59(6), 1383-1397. https://doi.org/10.1093/jxb/ern045 [details]
    • van den Burg, H. A., Tsitsigiannis, D. I., Rowland, O., Lo, J., Rallapalli, G., MacLean, D., ... Jones, J. D. G. (2008). The F-box protein ACRE189/ACIF1 regulates cell death and defense responses activated during pathogen recognition in tobacco and tomato. The Plant Cell, 20(3), 697-719. https://doi.org/10.1105/tpc.107.056978 [details]

    2007

    • Gabriels, S. H. E. J., Vossen, J. H., Ekengren, S. K., van Ooijen, G., Abd-El-Haliem, A. M., van den Berg, G. C. M., ... Joosten, M. H. A. J. (2007). An NB-LRR protein required for HR signalling mediated by both extra- and intracellular resistance proteins. Plant Journal, 50(1), 14-28. https://doi.org/10.1111/j.1365-313X.2007.03027.x [details]
    • van Ooijen, G., van den Burg, H. A., Cornelissen, B. J. C., & Takken, F. L. W. (2007). Structure and function of Resistance proteins in solanaceous plants. Annual Review of Phytopathology, 45, 43-72. https://doi.org/10.1146/annurev.phyto.45.062806.094430 [details]

    2006

    • Albrecht, M., & Takken, F. L. W. (2006). Update on the domain architectures of NLRs and R proteins. Biochemical and Biophysical Research Communications, 339(2), 459-462. https://doi.org/10.1016/j.bbrc.2005.10.074 [details]
    • Gabriels, S. H. E. J., Takken, F. L. W., Vossen, J. H., de Jong, C. F., Liu, Q., Turk, S. C. H. J., ... Joosten, M. H. A. J. (2006). cDNA-AFLP combined with functional analysis reveals novel genes involved in the hypersensitive response. Molecular Plant-Microbe Interactions, 19, 567-576. https://doi.org/10.1094/MPMI-19-0567 [details]
    • Takken, F. L. W., Albrecht, M., & Tameling, W. I. L. (2006). Resistance proteins: molecular switches of plant defence. Current Opinion in Plant Biology, 9, 383-390. https://doi.org/10.1016/j.pbi.2006.05.009 [details]
    • Takken, F. L. W., Tameling, W. I. L., Vossen, J. H., Albrecht, M., Berden, J. A., van Ooijen, G., & Cornelissen, B. J. C. (2006). The NB-ARC domain of R proteins acts as a molecular switch regulating plant innate immunity. In Q. C. Lópes-Lara Sánchez, S, & Geiger, O. (Eds.), Biology of Plant-Microbe Interactions. (pp. 225-230) [details]
    • Tameling, W. I. L., Vossen, J. H., Albrecht, M., Lengauer, T., Berden, J. A., Haring, M. A., ... Takken, F. L. W. (2006). Mutations in the NB-ARC domain of I-2 that impair ATP hydrolysis cause autoactivation. Plant Physiology, 140, 1233-1245. https://doi.org/10.1104/pp.105.073510 [details]

    2005

    • de la Fuente van Bentem, S., Vossen, J. H., de Vries, K. J., van Wees, A. C. M., Tameling, W. I. L., Dekker, H. L., ... Cornelissen, B. J. C. (2005). Heat shock protein 90 and its co-chaperone protein phosphatase 5 interact with distinct regions of the tomato I-2 disease resistance protein. Plant Journal, 43, 284-298. https://doi.org/10.1111/j.1365-313X.2005.02450.x [details]

    2004

    • Takken, F. L. W., van Wijk, R., Michielse, C. B., Houterman, P. M., Ram, A. F., & Cornelissen, B. J. C. (2004). A one-step method to convert vectors into binary vectors suited for Agrobacterium-mediated transformation. Current Genetics, 45, 242-248. https://doi.org/10.1007/s00294-003-0481-5 [details]

    2002

    • Luderer, R., Takken, F. L. W., de Wit, P. J. G. M., & Joosten, M. H. A. J. (2002). Cladosporium fulvum overcomes Cf-2-mediated resistance by producing truncated AVR2 elicitor proteins. Molecular Microbiology, 45(3), 875-884. https://doi.org/10.1046/j.1365-2958.2002.03060.x [details]
    • Tameling, W. I. L., Elzinga, S. D. J., Darmin, P. S., Vossen, J. H., Takken, F. L. W., Haring, M. A., & Cornelissen, B. J. C. (2002). The tomato R gene products I-2 and Mi-1 are functional ATP binding proteins with ATPase activity. The Plant Cell, 14, 2929-2939. https://doi.org/10.1105/tpc.005793 [details]
    • de Jong, C. F., Takken, F. L. W., Cai, X., de Wit, P. J. G. M., & Joosten, M. H. A. J. (2002). Attenuation of Cf-mediated defense responses at elevated temperatures correlates with a decrease in elicitor-binding sites. Molecular Plant-Microbe Interactions, 15(10), 1040-1049. https://doi.org/10.1094/MPMI.2002.15.10.1040 [details]
    • de Wit, P. J. G. M., Brandwagt, B. F., van den Burg, H. A., Cai, X., van der Hoorn, R. A. L., de Jong, C. F., ... Joosten, M. H. A. J. (2002). The molecular basis of co-evolution between Cladosporium fulvum and tomato. Antonie van Leeuwenhoek, 81, 409-412. https://doi.org/10.1023/A:1020553120889 [details]

    2019

    2010

    2008

    • Tameling, W. I. L., & Takken, F. L. W. (2008). Resistance proteins: Scouts of the plant innate immune system. In D. B. Collinge, L. Munk, & B. M. Cooke (Eds.), Sustainable disease management in a European context (pp. 243-255). Springer. https://doi.org/10.1007/978-1-4020-8780-6_4 [details]

    2005

    • Takken, F. L. W. (2005). Overeenkomsten en verschillen in ziekteresistentie tussen plant en dier. Gewasbescherming, 36, 262. [details]

    2004

    • Joosten, M. H. A. J., de Jong, C. F., de Wit, P. J. G. M., Takken, F. L. W., & Turk, S. C. H. J. (2004). Nucleotide sequences involved in plant resistance.

    2003

    • Turk, S. C. H. J., Takken, F. L. W., de Jong, C. F., Joosten, M. H. A. J., & de Wit, P. J. G. M. (2003). Nucleotide sequences involved in plant disease resistance.

    2002

    • Takken, F. L. W., & de Wit, P. J. G. M. (2002). Elicitor from Cladosporium.

    Talk / presentation

    • Takken, F. L. W. (speaker) (19-5-2011). The role of pathogen effectors in NLR-mediated plant innate immunity, International Meeting of the Collaborative Research Center SFB 648 “ Communication in Plants and their Responses to the Environment”, Halle (Saale), Germany.
    • Takken, F. L. W. (speaker) (8-10-2010). Molecular co-evolution in the tomato-Fusarium pathosystem, Justus Liebig University , Giessen, Germany, Giessen, Germany.
    • Takken, F. L. W. (invited speaker) (8-6-2010). Functional analysis of Fusarium oxysporum f.sp. lycopersici effector proteins., DFG funded colloquium: 'Microbial reprogramming of plant cell development' priority project SPP1212 'Plant-Micro', Freising, Germany.
    • Takken, F. L. W. (speaker) (11-5-2010). The arms race between effectors of Fusarium oxysporum and resistance proteins of tomato., Seminar series School of Biological and Biomedical Sciences, Durham University,, Durham, UK.
    • Takken, F. L. W. (speaker) (1-8-2009). How to resist a resistance protein?, British Society for Plant Pathology, Presidential Meeting 2009, Oxford, UK.
    • Takken, F. L. W. (speaker) (19-7-2009). How to resist tomato resistance proteins?, XIV International Congress on Molecular Plant-MicrobeInteractions, Quebec, Canada.
    • Takken, F. L. W. (speaker) (22-9-2008). Structure-function analysis of plant NB-LRR disease resistance proteins at the SFB 670, Structure Function and Evolution of Innate immunity, Max-Planck-Institut für Züchtungsforschung Koln, Germany.
    • Takken, F. L. W. (speaker) (9-9-2008). Resistance proteins: scouts of the plant innate immune system, 18th EUCARPIA congress, "Modern Variety Breeding for Present and Future Needs, Valencia, Spain.
    • Takken, F. L. W. (speaker) (25-6-2008). Resistance proteins: scouts of the plant innate immune system., 4th EPSO Conference, Presqu'ile de Giens (Côte d'Azur), France.
    • Takken, F. L. W. (speaker) (18-6-2008). Resistance proteins: scouts of the plant innate immune system., EPS/Bioexploit Summerschool “On the evolution of plant-pathogen interactions: from principles to practices, Wageningen, the Netherlands.
    • Takken, F. L. W. (speaker) (1-5-2008). Molecular aspects of I-2 mediated resistance to Fusarium oxysporum., Max-Planck-Institut für Züchtungsforschung, Koln, Germany.
    • Takken, F. L. W. (speaker) (10-2-2008). Function of the Nucleotide Binding Domain for R Proteins, Keystone Symposium on Plant Innate Immunity, Resort in Keystone, Colorado, USA.
    • Takken, F. L. W. (speaker) (7-12-2007). Molecular mechanisms regulating the activity of plant NB-LRR resistance proteins., seminar: Institute of Plant Biology., Zurich, Switzerland.
    • Takken, F. L. W. (speaker) (20-11-2007). The NB-ARC domain of R proteins acts as a molecular switch regulating plant innate immunity., seminar: Max von Pettenkofer Institut., LMU München, Germany.
    • Lukasik, E. (speaker), Cornelissen, B. J. C. (speaker) & Takken, F. L. W. (speaker) (17-10-2007). Role of nucleotide binding for R protein function., Bioexploit Meeting., Lunteren, the Netherlands.
    • Takken, F. L. W. (speaker) (12-7-2007). Host-Pathogen Interaction from Plants to Mammals -Distinct and Shared Pathways of Immune Defence., seminar: Inflammatory diseases of barrier organs., University Hospital, Kiel, Germany.
    • van Ooijen, G. (speaker), Cornelissen, B. J. C. (speaker) & Takken, F. L. W. (speaker) (1-1-2007). A role for the conserved MHD motif for R protein function., XIIIth International Congress on Molecular Plant-Microbe Interactions., Sorrento, Italy.
    • Takken, F. L. W. (speaker) (27-11-2006). The NB-ARC domain: an NTP-hydrolysing molecular switch., Comparative Biology of Innate Immune Systems, New York, USA.
    • Takken, F. L. W. (speaker), Tameling, W. I. L. (speaker), Albrecht, M. (speaker), van Ooijen, G. (speaker), de Vroomen, M. J. (speaker), de Vries, K. J. (speaker) & Cornelissen, B. J. C. (speaker) (13-8-2006). R proteins, molecular switches of plant defence., 8th Conference of the European Foundation for Plant Pathology & British Society of Plant Pathology Presedential Meeting, Copenhagen, Denmark.
    • Takken, F. L. W. (speaker) (2-2-2006). Innate immune receptors in plants and animals; do anlogous modules have comparable functions?, EPS Symposium Intracellular signalling, UvA, Amsterdam, The Netherlands.
    • Takken, F. L. W. (speaker), Tameling, W. I. L. (speaker), Vossen, J. H. (speaker), Albrecht, M. (speaker), Berden, J. A. (speaker), Haring, M. A. (speaker) & Cornelissen, B. J. C. (speaker) (14-12-2005). The NB-ARC domain of R proteins acts as a molecular switch regulating plant innate immunity., XII Int. congress on Molecular-Plant Microbe Interactions., Merida, Mexico.
    • Takken, F. L. W. (speaker) (27-4-2005). Overeenkomsten en verschillen in ziekteresistentie tussen plant en dier., Meeting: In gewasbeschermingsmanifestatie: Is het al tijd om te oogsten., Ede, The Netherlands.
    • Takken, F. L. W. (speaker) (27-4-2005). R proteins: molecular switches of disease resistance signalling., In 4e Gewasbeschermingsmanifestatie, Ede , The Netherlands.
    • Takken, F. L. W. (speaker) (4-4-2005). The role of nucleotide binding for R protein function., EPS Symposium, Lunteren, The Netherlands.
    • Takken, F. L. W. (speaker) (26-7-2004). R proteins: Molecular switches of disease resistance signalling., Invitation from the Boyce Thompson Institute at the Cornell University, Ithaca, United States.
    • Takken, F. L. W. (speaker) (6-4-2004). R proteins: molecular switches of disease resistance signalling., ALW/EPW Meeting, Lunteren, Nederland.
    • Takken, F. L. W. (speaker) (11-3-2004). R proteins: molecular switches of disease resistance signalling., 10th Nederland Biotechnology Conference, Ede, Nederland.
    • Takken, F. L. W. (speaker) (10-4-2003). Elucidation of the molecular and biochemical basis of I-2 mediated Fusarium resistance, Keystone Plant Biology: Functions and control of cell death - symposium, Snowbird, Utah, VS.
    • Takken, F. L. W. (speaker) (30-1-2003). The molecular and biochemical basis of I-2 mediated Fusarium resistance in tomato, WCS dat, Utrecht.

    2020

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  • Ancillary activities
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