This 8 day course is an intermediate level course for PhD students, postdocs and technicians in biology and biomedical science who already have experience with widefield and/or confocal fluorescence microscopy but want to extend their knowledge with the functional imaging techniques. After this course, participants will understand the principles of these methods and their limitations. In this course we will focus on functional imaging technologies that can be used in biology such as FRET, FLIM, FRAP, FCS & super-resolution microscopy. Furthermore, participants will be introduced to the latest developments in fluorescent probes, sample preparation, data-analysis and data statistics.