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Research core facilities

We are the Mass Spectrometry Research Core Facility of the Swammerdam Institute for Life Sciences. We apply mass spectrometry to a variety of biological questions from within the institute and our main focus areas are proteomics (bottom-up) and metabolomics (untargeted, LC-MS based). the Mass Spectrometry Core provides research support to scientists (SILS, FNWI, Amsterdam UMC or External) looking to apply metabolomics or proteomics analyses in their research. Contact us to discuss your project and the possibilities for the use of mass spectrometry.

Proteomics Facility of Amsterdam UMC

We also host the Proteomics Facility of the Amsterdam UMC, for project discussions please contact:

Dr. Dave Speijer
d.speijer@amsterdamumc.nl 
+31 (0) 20 - 5665134
Mo-We

Dr. D. (Dave) Speijer

Amsterdam UMC scientific liaison

 

Proteomics applications

We cover the large part of bottom up proteomics applications and also some limited intact protein analysis.

  • Protein Analytics: sequence confirmation, PTM-mapping, holo-mass determination, native spray mass spectrometry.
  • Quantitative Proteomics (Label-Free, SILAC/15N metabolic, Isobaric chemical labelling).
  • Proteome Wide Translation and Degradation rates (Pulsed SILAC/TMT, Pulsed SILAC-AHA, Hyper-pulsed AHA-SILAC).
  • Deep phospho-proteome analysis (under development) .
  • Protein interactions: CO-IP, CHIP-SICAP, IP-MS, Thermal Proteome Profiling.
  • Low sample input proteomics for rare cells or small tissue sections (under development).

Metabolomics applications

As mass spectrometrists we routinely measure small molecules, our focus is untargeted quantitative LC-MS based metabolomics and lipidomics.

  • Untargeted metabolomic profiling of polar and apolar metabolites.
  • Untargeted lipidomic profiling (under development).
  • Targeted metabolomic profiling of large metabolite sets (under development).
  • Low  sample input metabolomics (under development).