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Using structure-guided molecular evolution and advanced microscopy techniques, dr.ir. Mark A. Hink, ing. Linda Joosen & prof. dr. Theodorus W.J. Gadella Jr. of the section of Molecular Cytology of the Swammerdam Institute for Life Sciences (SILS) in collaboration with the group of prof. Verkusha, scientist at the Albert Einstein College of Medicine in New York, have created, characterized and applied a new fully genetically encoded orange fluorescent protein LSSmOrange

The unique property LSSmOrange is the extremely large shift between the wavelength (color) at optimal excitation (blue 437 nm) and the color of the induced fluorescence (orange, 572 nm). This so called large Stokes shift (LSS) is highly advantageous for the study of molecular interactions or cellular processes with fluorescent biosensors.

For the complete article see link; View JACS

View JACS

Research group Molecular Cytology