Course: FEBS practical course

Symposium

Advanced imaging of molecular complexes inside living cells

 

Molecular complexes play an important role in cellular processes like transcription, receptor signaling and cytoskeletal structure formation. Malfunction of the individual protein components and their interplay may result in disease. Microscopic techniques can be used to elucidate complex composition, organization and dynamics within the living cell.

This course is organized by the Leeuwenhoek Centre for Advanced Microscopy (LCAM) and provides an in-depth view of specific advanced imaging techniques used at imaging centers of AMC, NKI and FNWI. The course focuses fully on intensive hands-on practical sessions and interactive discussions with experts in the various microscope techniques.

This practical advanced course aims to provide 20 young scientists in this field with theoretical background and to give hands-on experience of state-of-the-art microscopy techniques.

Techniques:

• Confocal microscopy
• Spinning disc and TIRF microscopy
• Super-Resolution Microscopy
• Förster resonance energy transfer (FRET)
• Fluorescence lifetime imaging (FLIM)
• Fluorescence recovery after Photobleaching (FRAP)
• Fluorescence Correlation Spectroscopy (FCS)

 

Target audience: PhD students and postdocs. Experience with microscopy is a prerequisite for this Advanced Microscopy Course.

 

 

 

Published by  Swammerdam Institute